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Varicocele repair (varicocelectomy) is indicated in infertile men with large varicoceles that are readily apparent on physical exam, and improves semen parameters in 60–70% of these patients

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Concentrate the cleaved unbound protein to a final volume that is 0.5 to 1% of the column volume to be used for the separation using an ultrafiltration device, such as Amicon Ultra (Millipore), according to the manufacturer’s instructions (see Note 37)