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The epithelial layer was then treated with 100 U/mL collagenase type I (Wako, Osaka, Japan) for 80 min and 0.25% trypsin (Thermo Fisher Scientific, Waltham, MA, USA) for 10 min at 37 °C

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Using regular sterile water (no preservative), shaking instead of swirling, or storing at room temp degrades the peptide fast

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