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Kicqstart primers for TBP, KEAP1, NFE2L2, SRXN1 and SQSTM1 (Sigma Aldrich, Zwijndrecht, The Netherlands) were used to detect mRNA levels using POWRUP SYBR master mix (Thermo Scientific, Leiden, The Netherlands)

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This is one of the strongest arguments for staying at or near the 100 mcg per injection saturation dose rather than escalating higher