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Statistical Analysis Statistical analysis was performed by parametric one-way analysis of variance (ANOVA), with post-hoc Newman–Keuls test and non-parametric Kruskal–Wallis test and subsequently the Mann–Whitney U test to compare groups

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Moreover, compared with WT serum-treated group, GCGR-KO serum-treated islets showed increased glucagon secretion under both normal (7 mM glucose) and low glucose (1 mM glucose) conditions (Fig