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Published research describes the following mechanisms for MC1R agonists like afamelanotide: NF-kappaB suppression — reduced transcription of pro-inflammatory genes Cytokine modulation — decreased TNF-alpha, IL-1beta, and IL-6 production Nitric oxide regulation — normalization of NO pathway signaling Adhesion molecule downregulation — reduced inflammatory cell migration IL-10 upregulation — increased anti-inflammatory cytokine output These effects have been demonstrated in vitro and in animal models of acute, chronic, and systemic inflammation (Catania et al., 2004)

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