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Minoxidil still has the deepest evidence base for non-scarring alopecia, with multiple randomized controlled trials showing 5% topical formulations producing meaningful regrowth over 48 weeks (Olsen et al., 2002, Journal of the American Academy of Dermatology)

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The leachate of hydrogels was obtained by completely sterilizing each group of hydrogels and immersing them in serum-free culture medium