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The RT-qPCR analyses were performed in a LightCycler® 480 II detection system (Roche Diagnostics GmbH, Roche Applied Science, Mannheim, Germany) under the following thermal cycler conditions: initial denaturation for 5 min at 95°C, followed by 45 cycles for 15 sec at 95°C, 15 sec at 60°C and 15 sec at 72°C, using the primers listed in Tables 1 and 2
Carnitine occurs naturally in the body and supports fat metabolism